Journal: Materials Today Bio
Article Title: Sequential delivery of sinigrin and dabigatran by an in situ self-stabilizing dynamic hydrogel attenuates intervertebral disc degeneration
doi: 10.1016/j.mtbio.2026.102827
Figure Lengend Snippet: Biocompatibility of the CAD-gel and DS@CAD. (A) Experimental flowchart of the MTT assay and live/dead staining (created with BioRender.com ). (B) Live/dead staining images of rat NPCs after different interventions at 24 h, 48 h, and 72 h (n = 3). (C–E) Quantitative analysis of live/dead staining at 24 h, 48 h and 72 h showing the percentage of live and dead cells. (F) Phalloidin staining images of NPCs under different treatments. (G) MTT assay results for rat NPCs following various treatments (n = 3). (H) Flow cytometric analysis of apoptosis in rat NPCs after treatment (n = 3). (I) Percentage of apoptotic NPCs after different treatments. (J) Experimental workflow for the In Vivo Imaging System (IVIS) (created using BioRender.com ). (K) IVIS images demonstrating degradation of the cy5-labelled CAD-gel in IVDs over time (n = 5).
Article Snippet: Fluorescently labelled hydrogels were stereotactically delivered into rat intervertebral discs, and their in vivo degradation kinetics were monitored longitudinally on postoperative days 0, 3, 7, 14, and 28 via noninvasive IVIS Spectrum preclinical imaging (PerkinElmer), with fluorescence intensity decay at the implantation site correlated with degradation.
Techniques: MTT Assay, Staining, In Vivo Imaging